From key visible wavelengths to high pulse energy, Mobius Photonics develops fiber-based lasers with capabilities tailored for super-resolution microscopy applications.
Super-resolution microscopy is evolving quickly and microscopists are demanding new capabilities from laser sources. At the forefront of the field, scientists are imaging living, intact organisms beyond the diffraction barrier using fluorescent proteins – the green fluorescent protein (GFP), the yellow fluorescent protein (YFP) and their variants. These innovators require lasers in the visible with sufficient pulse energy to obtain resolutions on the order of tens of nanometers.*
Mobius has developed fiber-based laser systems with proven specifications for far-field imaging on the nanoscale*†. Our unique systems provide intense light at discrete wavelengths in a spectral range critical for de-exciting fluorescent markers, particularly workhorse fluorescent proteins. They also feature repetition rates high enough to allow fast imaging.
Critical spectral range.
Our newest laser system emits intense light between 532 and 654 nm – ideal for de-exciting key fluorescent proteins.
Multiple lines from one laser.
Our systems produce several discrete visible lines; wavelength can be switched on the fly.
High spectral density.
Producing more than 20 nanojoules per nanometer per pulse, Mobius systems provide a narrow spectral window while still preserving high pulse energy.
High pulse energy.
Our lasers' pulse energy for a single color exceeds 20 nanojoules per pulse, enabling ultra-high-res image generation.
~1 nanosecond pulses.
Our systems' pulse duration is a good match to the fluorescence lifetimes of many markers.
High repetition rates.
Featuring repetition rates as high as 20 MHz, our systems are made for rapid image acquisition.
To learn more about our lasers for super-resolution microscopy, please contact email@example.com.
*Rankin, B. R., G. Moneron, C. A. Wurm, J. C. Nelson, A. Walter, D. Schwarzer, J. Schroeder, D. A. Colón-Ramos, S. W. Hell: "Nanoscopy in a Living Multicellular Organism Expressing GFP" Biophys. J., 100 (12), L63 - L65.
†Rankin, B. R., S. W. Hell (2009): "STED microscopy with a MHz pulsed stimulated-Raman-scattering source". Opt. Exp. 17 (18), 15679 - 15684.